Quantitative Biology · 2014 · 366 citations · 67 references
Life SciencesGeneticsMolecular BiologyGenetic EngineeringGenome EditingGenome EngineeringCrispr-based EngineeringCrispr-cas SystemsGuide Rna DesignOff-target EffectsTarget Site SelectionCas Nuclease SpecificityCas9 Off-target ActivityCrispr-cas Genome EditingCrispr-cas Target CleavageCrispr-cas9 Genome EditingOff-target Effect AnalysisRna TargetingTarget Site Specificity
The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.
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