Concepedia

Guide Rna Design

Guide RNA Design is the systematic process of selecting and constructing a single RNA sequence that directs a genome-editing nuclease to a specific genomic target by combining a scaffold sequence with a customizable spacer region complementary to the DNA site of interest.

1.3K

Publications

251.7K

Citations

6.7K

Authors

1.4K

Institutions

Publications per year

2017–2026

863

Variants

Grna Design

Parents

Children

Publications

1.3K

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong, F. Ann Ran, David Cox et al. · Science · 2013 · 15.7K citations

, , +16

Genome engineering using the CRISPR-Cas9 system

F. Ann Ran, Patrick D. Hsu, Jason Wright et al. · Nature Protocols · 2013 · 11.7K citations · Full text

, , +14

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Lei S. Qi, Matthew H. Larson, Luke A. Gilbert et al. · Cell · 2013 · 5.2K citations · Full text

, , +16

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench, Nicolò Fusi, Meagan E. Sullender et al. · Nature Biotechnology · 2016 · 5K citations

, , +13

DNA targeting specificity of RNA-guided Cas9 nucleases

Patrick D. Hsu, David A. Scott, Joshua A. Weinstein et al. · Nature Biotechnology · 2013 · 4.9K citations · Full text

, , +10

1–5 of 1.3K

Authors

6.7K

  1. FZ
    Feng Zhang

    Broad Institute, United States

    27 publications · 58.4K citations

    27

    h-index

  2. JK
    J. Keith Joung

    Massachusetts General Hospital, United States

    29 publications · 16.9K citations

    24

    h-index

  3. JA
    Jennifer A. Doudna

    University of California, Berkeley, United States

    23 publications · 13K citations

    21

    h-index

  4. EV
    Eugene V. Koonin

    National Center for Biotechnology Information, United States

    16 publications · 13.6K citations

    15

    h-index

  5. DR
    David R. Liu

    Harvard University, United States

    13 publications · 3.5K citations

    13

    h-index

1–5 of 6.7K

Institutions

1.4K

  1. Harvard University

    Cambridge, United States

    64 h-index

    393 publications

    269.7K citations

  2. Howard Hughes Medical Institute

    Chevy Chase, United States

    55 h-index

    224 publications

    128.9K citations

  3. Broad Institute

    Cambridge, United States

    51 h-index

    313 publications

    389.5K citations

  4. Massachusetts Institute of Technology

    Cambridge, United States

    46 h-index

    241 publications

    359.6K citations

  5. Chinese Academy of Sciences

    Beijing, China

    35 h-index

    261 publications

    44.2K citations

  6. Massachusetts General Hospital

    Boston, United States

    34 h-index

    220 publications

    137.6K citations

  7. University of California, Berkeley

    Berkeley, United States

    31 h-index

    202 publications

    63.9K citations

  8. McGovern Institute for Brain Research

    Cambridge, United States

    31 h-index

    124 publications

    347.7K citations

1–8 of 1.4K

Venues

  1. Nature Biotechnology

    71 publications · 48.8K citations

    66

    h-index

  2. Nature Communications

    55 publications · 7.2K citations

    42

    h-index

  3. Nucleic Acids Research

    58 publications · 12.1K citations

    38

    h-index

  4. Molecular Cell

    44 publications · 7.4K citations

    36

    h-index

  5. Nature

    35 publications · 28.8K citations

    29

    h-index

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