A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jínek, Krzysztof Chylinski, Ines Fonfara, +3

Science

Crispr RnasAdaptive Bacterial ImmunityCrispr-associated Protein Cas9MedicineGenetics +13

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong, F. Ann Ran, David Cox, +8

Science

Single Crispr ArrayEngineeringShort RnasGeneticsSynthetic Biology +10

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali, Luhan Yang, Kevin M. Esvelt, +5

Science

EngineeringGeneticsMolecular BiologyCrispr ComponentsMultiple Grnas +14

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

Rodolphe Barrangou, Christophe Fremaux, Hélène Deveau, +5

Science

Phage-resistance PhenotypeGeneticsBacteriophageSpacer-phage Sequence SimilarityAntimicrobial Resistance +10

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Lei S. Qi, Matthew H. Larson, Luke A. Gilbert, +4

Cell

Off-target EffectEngineeringRna-guided PlatformGenetic EngineeringMolecular Biology +7

DNA targeting specificity of RNA-guided Cas9 nucleases

Patrick D. Hsu, David A. Scott, Joshua A. Weinstein, +11

Nature Biotechnology

Molecular BiologyGene EditingGenome EditingRna-guided Cas9 NucleasesMedicine +1

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

Luke A. Gilbert, Matthew H. Larson, Leonardo Morsut, +10

Cell

Transcriptional RegulationNatural SciencesMolecular BiologyGene EditingGenome Editing +4

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

Haoyi Wang, Hui Yang, Chikdu Shivalila, +4

Cell

Off-target EffectEngineeringGenome SurgeryGeneticsCrispr/cas-mediated Genome Engineering +10

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

F. Ann Ran, Patrick D. Hsu, Chie-Yu Lin, +8

Cell

Rna-guided Crispr Cas9GeneticsMolecular BiologyGenetic EngineeringGene Editing +5

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

Yanfang Fu, Jennifer A Foden, Cyd Khayter, +4

Nature Biotechnology