Hyper-acidic protein fusion partners improve solubility and assist correct folding of recombinant proteins expressed in Escherichia coli

<i>Escherichia coli</i> maltose‐binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

Rachel B. Kapust, David S. Waugh · Protein Science · 1999 · 964 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +16

Ulp1-SUMO Crystal Structure and Genetic Analysis Reveal Conserved Interactions and a Regulatory Element Essential for Cell Growth in Yeast

Elena Mossessova, Christopher D. Lima · Molecular Cell · 2000 · 710 citations · Full text

Life Sciences, Genetics, Molecular Biology +11

SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins

Michael P. Malakhov, Michael R. Mattern, Oxana A. Malakhova et al. · Journal of Structural and Functional Genomics · 2004 · 550 citations

Life Sciences, Biochemistry, Molecular Biology +11

Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO

Jeffrey G. Marblestone, Suzanne C. Edavettal, Yiting Lim et al. · Protein Science · 2005 · 506 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +16

The nucleotide sequence of pACYC184

Ronald E. Rose · Nucleic Acids Research · 1988 · 408 citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +12