Concepedia

Solubility Engineering

Solubility Engineering is a protein engineering strategy within the broader field of Protein Expression Engineering that specifically focuses on modifying amino acid sequences or expression conditions to enhance the solubility of recombinant proteins, particularly antibody fragments, during heterologous expression systems.

118

Publications

7K

Citations

591

Authors

194

Institutions

Publications per year

2017–2026

34

Parents

Publications

118

<i>Escherichia coli</i> maltose‐binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

Rachel B. Kapust, David S. Waugh · Protein Science · 1999 · 964 citations · Full text

, , +16

The CamSol Method of Rational Design of Protein Mutants with Enhanced Solubility

Pietro Sormanni, Francesco A. Aprile, Michele Vendruscolo · Journal of Molecular Biology · 2014 · 527 citations

, , +14

Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners

Sreedevi Nallamsetty, David S. Waugh · Protein Expression and Purification · 2005 · 189 citations

, , +16

Structure-based engineering of a monoclonal antibody for improved solubility

Sheng‐Jiun Wu, Jinquan Luo, Karyn T. O’Neil et al. · Protein Engineering Design and Selection · 2010 · 183 citations

, , +17

Engineering soluble proteins for structural genomics

J.D. Pédelacq, Emily Piltch, E.C. Liong et al. · Nature Biotechnology · 2002 · 179 citations

, , +12

1–5 of 118

Authors

591

  1. DS
    David S. Waugh

    National Cancer Institute, United States

    6 publications · 1.4K citations

    6

    h-index

  2. GS
    Geoffrey S. Waldo

    Los Alamos National Laboratory, United States

    3 publications · 295 citations

    3

    h-index

  3. MU
    Mathias Uhlén

    KTH Royal Institute of Technology, Sweden

    3 publications · 156 citations

    3

    h-index

  4. JD
    Jeffrey D. Fox

    National Cancer Institute, United States

    3 publications · 364 citations

    3

    h-index

  5. TC
    Thomas C. Terwilliger

    Los Alamos National Laboratory, United States

    3 publications · 295 citations

    3

    h-index

1–5 of 591

Institutions

194

  1. Frederick National Laboratory for Cancer Research

    Frederick, United States

    6 h-index

    14 publications

    1.6K citations

  2. National Cancer Institute

    Bethesda, United States

    6 h-index

    13 publications

    1.4K citations

  3. Center for Cancer Research

    Bethesda, United States

    5 h-index

    8 publications

    1.6K citations

  4. KTH Royal Institute of Technology

    Stockholm, Sweden

    4 h-index

    14 publications

    829 citations

  5. State Key Laboratory of Food Science and Technology

    Wuxi, China

    3 h-index

    12 publications

    1.1K citations

  6. Yangzhou University

    Yangzhou, China

    3 h-index

    11 publications

    514 citations

  7. Universitat Autònoma de Barcelona

    Cerdanyola del Vallès, Spain

    3 h-index

    15 publications

    976 citations

  8. Los Alamos National Laboratory

    Los Alamos, United States

    3 h-index

    17 publications

    2K citations

1–8 of 194

Venues

  1. Food Chemistry

    6 publications · 350 citations

    6

    h-index

  2. Protein Expression and Purification

    6 publications · 439 citations

    6

    h-index

  3. Protein Engineering Design and Selection

    PEDS · 6 publications · 340 citations

    6

    h-index

  4. Journal of Biotechnology

    5 publications · 166 citations

    5

    h-index

  5. Protein Science

    PS · 5 publications · 1.4K citations

    5

    h-index

1–5