Foods · 2014 · 64 citations · 23 references
Life SciencesChemistryFood ScienceAnalytical ChemistryFood AnalysisMetabolomicsExtraction TechniquesMagnetic Resonance SpectroscopyMetabolite ExtractionMetabolite IdentificationMicrowave SpectroscopyNuclear Magnetic Resonance MetabolomicsExtraction Time OptimizationGeographical Origin DeterminationMetabolite Extraction ProtocolsMicrowave-assisted ExtractionMicrowave-assisted Sample PreparationNuclear Magnetic Resonance Spectroscopy Data AnalysisNuclear Magnetic Resonance Spectroscopy-based Metabolite ProfilingQuantitative Nmr Spectroscopy
An NMR analytical protocol is proposed to characterize saffron samples of different geographical origin (Greece, Spain, Hungary, Turkey and Italy). A microwave-assisted extraction procedure was developed to obtain a comparable recovery of metabolites with respect to the ISO specifications, reducing the solvent volume and the extraction time needed. Metabolite profiles of geographically different saffron extracts were compared showing significant differences in the content of some metabolites.
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