A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jínek, Krzysztof Chylinski, Ines Fonfara, +3

Science

Crispr RnasAdaptive Bacterial ImmunityCrispr-associated Protein Cas9MedicineGenetics +13

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong, F. Ann Ran, David Cox, +8

Science

Single Crispr ArrayEngineeringShort RnasGeneticsSynthetic Biology +10

Enzymatic assembly of DNA molecules up to several hundred kilobases

Daniel G. Gibson, Lei Young, Ray-Yuan Chuang, +3

Nature Methods

Dna NanotechnologyDna SequencingDnaEngineeringBiochemistry +7

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali, Luhan Yang, Kevin M. Esvelt, +5

Science

EngineeringGeneticsMolecular BiologyCrispr ComponentsMultiple Grnas +14

Efficient genome editing in zebrafish using a CRISPR-Cas system

Woong Y. Hwang, Yanfang Fu, Deepak Reyon, +6

Nature Biotechnology

Genome SurgeryGeneticsEfficient Genome EditingGenetic EngineeringGene Editing +3

Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Giedrius Gasiūnas, Rodolphe Barrangou, Philippe Horvath, +1

Proceedings of the National Academy of Sciences

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

Stan J. J. Brouns, Matthijs M. Jore, Magnus Lundgren, +7

Science

Crispr RnasViral ReplicationOff-target EffectCrispr RnaNatural Sciences +11

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

Elitza Deltcheva, Krzysztof Chylinski, Cynthia M. Sharma, +6

Nature

Natural SciencesGeneticsCrispr Rna MaturationRna BiologyMolecular Biology +7

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Seung Woo Cho, Sojung Kim, Jong Min Kim, +1

Nature Biotechnology

Human CellsMedicineNatural SciencesSynthetic BiologyMolecular Biology +10

Phage Response to CRISPR-Encoded Resistance in <i>Streptococcus thermophilus</i>

Hélène Deveau, Rodolphe Barrangou, Josiane E. Garneau, +6

Journal of Bacteriology