Transient Expression Assay in Strawberry Fruits

Abstract

Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, virus-induced gene silencing (VIGS) and Agrobacterium tumefaciens-mediated transformation in fruit have been widely used as the transient expression approaches. Unlike VIGS, the latter one can be utilized not only for gene knock-down, but also for overexpression and knock-out.