Cell Reports · 2018 · 16 citations · 29 references
Life SciencesImmunologyMolecular BiologyMolecular ImmunologyMolecular VirologyVirologyAntigen RecognitionMajor Histocompatibility ComplexSecretory PathwayT Cell BiologyViral InfectionsViral TropismCd8 Positive T CellsMhc Class I MoleculesMhc Class I TraffickingSecretory Pathway RegulationTransmembrane ProteinsViral Evasion MechanismsViral Immune Evasion MechanismsViral Immune Evasion Signaling
The murine cytomegalovirus immunoevasin m152/gp40 binds major histocompatibility complex (MHC) class I molecules and retains them in the early secretory pathway by a previously unknown mechanism, preventing antigen presentation to CD8+ T cells. We show that retention of class I and of gp40 itself depends on a lumenal linker sequence in gp40. With unbiased co-immunoprecipitation and mass spectrometry, we find that, through this linker, gp40 binds to TMED10/Tmp21/p24δ1, a member of the p24 family of endoplasmic reticulum (ER)/Golgi transmembrane proteins. We show that the C-terminal KKxxx Golgi-to-ER retrieval signal of TMED10 is required for gp40-mediated retention of class I. We thus identify a viral interaction partner of the p24 proteins and their exploitation for viral immune evasion.
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In-gel digestion for mass spectrometric characterization of proteins and proteomes
Andrej Shevchenko, Henrik Tomas, J. Havli et al. · Nature Protocols · 2006 · 5.1K citations