Publication | Open Access
Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.
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1981
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HistocompatibilityAnti-iab AntibodiesHla ImmunogeneticsImmunologyPathologyImmunodominanceMhc AntigensAntigen ProcessingImmunotherapyImmunogeneticsImmunochemistryAntibody EngineeringAutoimmune DiseaseAllergyHuman Leukocyte AntigenAutoimmunityHumoral ImmunityH-2b HaplotypeIsotype ExpressionHla TypingImmunoglobulin EMonoclonal AntibodiesMedicineHybridoma Antibodies
Eleven hybridoma antibodies directed against mouse MHC products of the H‑2b haplotype were produced and characterized, and an isotype analysis of conventional cytotoxic alloantisera from the same strain combinations was performed. Seven hybridoma antibodies reacting to H‑2Kb/H‑2Db antigens cross‑reacted with other H‑2 antigens, with five lacking known specificity, while four anti‑Iab antibodies targeted I‑A subregion antigens and some displayed private specificities and fine determinant discrimination; a high frequency of IgM‑secreting hybridomas was observed in C3H→C3H.SW immunizations versus IgG dominance in the reverse, and isotype analysis of alloantisera confirmed the same pattern, suggesting H‑2‑linked control of isotype expression.
Eleven hybridoma antibodies directed against mouse major histocompatibility complex products of the H-2b haplotype have been produced and characterized. Of 7 antibodies reacting to H-2Kb and/or H-2Db antigens, all cross-reacted with other H-2 antigens, and 5 exhibited no correspondence with a known H-2 specificity established in the H-2 chart. Four anti-Iab antibodies all reacted with antigens encoded by the I-A subregion. Some of these antibodies showed no cross-reaction with other haplotypes, indicating reactions to private specificities of the I-Ab antigen. In addition, these anti-Ia antibodies appeared to be capable of distinguishing fine determinant differences, which corresponding alloantisera failed to reveal. A high frequency of hybridomas secreting IgM antibodies was found after fusions of spleen cells obtained from C3H anti-C3H.SW immunized mice, in contrast to the dominance of IgG hybridomas produced previously by fusions of spleen cells from mice immunized in the reverse direction. An isotype analysis of conventional cytotoxic alloantisera from the same strain combinations was therefore performed. The same correlation with respect to isotype expression was found, indicating that hybridoma antibodies reflect normal antibody responses and suggesting H-2-linked control of this expression.