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Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production

EngineeringEndogenous Crispr-cas SystemMultiplex Genome EditingBioengineeringBiotechnologyMolecular BiologyGenetic EngineeringSynthetic BiologyHigh-level Butanol ProductionGenome EngineeringGene EditingMicrobiologyGenome EditingOff-target EffectMedicineCrispr