Rapid identification of α-glucosidase inhibitors from Dioscorea opposita Thunb peel extract by enzyme functionalized Fe₃O₄ magnetic nanoparticles coupled with HPLC-MS/MS

Abstract

= 2.55 mM and 0.40 mM, respectively) were significantly higher than that of acarbose (as control). Taking advantage of the specificity in enzyme binding and the convenience of magnetic separation, this method has great potential for rapid and fast screening of α-glucosidase inhibitors from complex natural resources.