Distribution of Polyphenol Oxidase in Flour Millstreams of Canadian Common Wheat Classes Milled to Three Extraction Rates

Abstract

CONCLUSIONS The availability of an assay kit for the determination of starch damage in flour offers a convenient and simple alternative to the current standard methods. Starch damage determinations with the assay kit are highly correlated to those of the standard methods, but the kit procedure is standardized and more rapid. With the kit procedure, 40 samples can be analyzed in 2 hr. The kit is therefore applicable for use in situations where there are large sample numbers, such as in the monitoring of millstream runs and in wheat breeding programs. The starch damage assay kit is now available commercially from MegaZyme (Aust) Pty Ltd. ACKNOWLEDGMENTS We thank all of the laboratories that participated in the collaborative evaluation of the starch damage assay kit. We also thank J. R. Donelson of the U.S. Department of Agriculture Agricultural Research Station Soft Wheat Quality Laboratory, Wooster, for the provision of starch damage results by the AACC and Wooster methods and A. Evers of the Flour Millers and Bakers Research Association, Charleywood, for distributing the assay kits to collaborators in the UK. We also thank Malcolm Glennie-Holmes, Bill Barnes, David Mugford, and Arthur Gilmour for their comments on the manuscript. This research was funded in part by the Grains Research Development Corporation of Australia.