A Simple and Rapid HPLC Method for Analysis of Vitamin-C in Local Packed Juices of Pakistan

Abstract

3 Abstract: The growing number of various tetra packed juices industries requires reliable quality control to insure the actual amount of ingredient and additives reported on the labels of packed juices by the companies. Here our research related to the development and validation of a simple and rapid RP-HPLC method to estimate the actual amount of Vitamin-C present in packed juices. It was estimated by Hitachi D-2000 Elite HPLC system manager using gradient pump system, separation was carried out by using C-18 column and detection by UV-Visible Detector. The retention time was within 1.63-1.65 minutes. Standard curves were linear over the concentration range of 0.1 to 2.5mg/ml. The extraction recovery was within 94 and 101%. The proposed method was found to be rapid, accurate, repeatable and consistent. This method was also compared with oxidation- reduction methods using standardized 2,6-Dichloroindophenol (DCP), to quantifying verifying ascorbic acid levels. We observed that the amounts of ascorbic acid reported by the two methods were mostly same and identical. In the result we found that Most of our local tetra packed juices companies may underestimate or overestimate the actual content of vitamin-C.