Discovery of an ovine α_s2-casein variant

Abstract

A novel ovine alpha S2-casein variant has been detected using discontinuous polyacrylamide gel electrophoresis at alkaline pH, two dimensional electrophoresis and immunoblotting. It is characterized by a greater negative net charge and a lower isoelectric point compared with the most common ovine alpha S2-casein variant. The phenotypic frequency in the Manchega breed is 5.5%.