Effect of Physicochemical Parameters on the Separation of Proteins from Human Placental Extract by Using a Continuous Foam Fractionating Column

Abstract

Abstract The recovery of valuable proteins (like proteolytic enzymes) from nonconventional biological sources by using modern separation techniques is becoming increasingly important because of their high commercial potential. In the present investigation, isolation of proteins from human placental extract by using the continuous foam fractionation technique is studied. The effect of different physicochemical parameters on the separation of proteins and purification of proteolytic enzymes are reported. A method for finding a quasi-equilibrium relationship between the solute concentration in the foamate and in the effluent by using the same system is described. The quasi-equilibrium curve is also presented.