Applied and Environmental Microbiology · 2003 · 379 citations · 29 references
AgricultureMicrobiologyPoultry ScienceBacteriologyPoultry MicrobiologyPoultry PathologyBacterial Diseases Of PoultryFoodborne PathogensMicrobial ContaminationPoultry BacteriologyPoultry Infectious DiseasesCampylobacter InfectionCampylobacter ReductionControl Of Bacterial DiseasesPathogen Exclusion EfficacyPrevention Of Bacterial DiseasesSalmonella Control In PoultrySalmonella ReductionSalmonella Resistance
Lytic bacteriophages, applied to chicken skin that had been experimentally contaminated with Salmonella enterica serovar Enteritidis or Campylobacter jejuni at a multiplicity of infection (MOI) of 1, increased in titer and reduced the pathogen numbers by less than 1 log(10) unit. Phages applied at a MOI of 100 to 1,000 rapidly reduced the recoverable bacterial numbers by up to 2 log(10) units over 48 h. When the level of Salmonella contamination was low (< log(10) 2 per unit area of skin) and the MOI was 10(5), no organisms were recovered. By increasing the number of phage particles applied (i.e., MOI of 10(7)), it was also possible to eliminate other Salmonella strains that showed high levels of resistance because of restriction but to which the phages were able to attach.
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