Ion‐Exchange Chromatographic Determination of Lysozyme in Egg White

Abstract

ABSTRACT Pure lysozyme solutions were chromatographed on diethylaminoethyl (DEAE) cellulose then monitored spectrophotometrically and by biological activity measurement. Although some biological activity was lost during chromatography, standard curves relating enzyme content to chromatographic peak area corrected for this loss. The experimental system produced egg white lysozyme concentrations which were comparable to literature values. The method of determination could be used in the comparison of total enzyme concentration to active enzyme in experimental egg products.