Experimental Biology and Medicine · 1965 · 807 citations · 0 references
Life SciencesBiochemistryEnzyme TechnologyEnzymologyProtein BiochemistryAssay DevelopmentBioanalytical ChemistryEnzyme AssaysEnzyme CharacterizationEnzyme DiagnosticsEnzyme KineticsAssayAssay SensitivityEnzymatic AssaysEnzyme Activity AssaysEnzyme Assay DevelopmentEnzyme Assay KineticsEnzyme Kinetics AssaysSpectrophotometric Enzyme Assays
An improved method has been developed for assay of muramidase in several biological systems. This method involves measurement of the rate of lysis of a 25 mg% suspension of M. lysodeikticus in 0.05 M NaCl and M/15 phosphate buffer, pH 6.2. The rate of lysis is measured in a Beckman DB spectrophotometer at 540 mμ with an attached recorder. This modification allows continuous measurement of the initial reaction velocity for samples containing from 0.1 to 10 μg of muramidase.