Cell Biology International · 2009 · 22 citations · 35 references
Life SciencesCell BiologyGeneticsApoptosisCarcinogenesisInflammationDna Damage SignalingDna Damage-induced ApoptosisGenome InstabilityGenomic InstabilityGenotoxic CarcinogenesisMicrosatellite InstabilityChromosomal ImbalanceCytogenetic AbnormalitiesDna Damage InductionDna Damage Response PathwaysDna Damage ResponsesGenotoxic Stress ResponseInflammatory Cytokine ProductionMicrosatellite Instability Analysis
The toxic response of cultured human colon epithelial-FHC cells to methyl isocyanate was investigated with regard to genomic instability. Qualitative and quantitative assessments of the extent of phosphorylation of DNA damage signaling factors such as ATM, gammaH2AX and p53, was increased in treated cells compared to controls. At the same time, many treated cells were arrested at the G2/M phase of the cell cycle, and had an elevated apoptotic index and increased inflammatory cytokine levels. Cytogenetic analyses revealed varied chromosomal anomalies, with abnormal expression of pericentrin protein. Analysis through ISSR PCR demonstrated increased microsatellite instability. The results imply that isocyanates can cause genomic instability in colonocytes.
35
Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis.
Marc S. Greenblatt, W P Bennett, Monica Hollstein et al. · PubMed · 1994 · 3.5K citations
Centrosome abnormalities and chromosome instability occur together in pre-invasive carcinomas.
Germán Pihán, Jan Wallace, Yening Zhou et al. · PubMed · 2003 · 376 citations · Full text