Journal of Applied Microbiology · 1998 · 49 citations · 15 references
AgricultureFood ScienceMicrobiologyDiagnostic MicrobiologyFood MicrobiologyFood SafetyFoodborne Pathogen DetectionMicrobial Food SafetyMolecular DiagnosisPathogen DetectionPathogen Detection In FoodsPolymerase Chain ReactionPcr-based DetectionPolymerase Chain Reaction (Pcr) AmplificationPolymerase Chain Reaction (Pcr) AssaysPolymerase Chain Reaction (Pcr) DiagnosisPolymerase Chain Reaction (Pcr) DiagnosticsPolymerase Chain Reaction (Pcr)-based IdentificationPolymerase Chain Reaction-based DiagnosticsPolymerase Chain Reaction-restriction Analysis
A primer set of oligonucleotides (Salm 3 and Salm 4) from the invA gene of Salmonellae has been evaluated for the specific detection of Salmonella spp. by the polymerase chain reaction (PCR). This primer set amplified 33 Salmonella serovars but did not amplify 16 non-Salmonella bacteria. Moreover, after PCR amplification, it was possible to identify Salm. typhimurium by restriction enzyme analysis. The PCR-RE method developed could represent a helpful tool for detecting Salmonella spp., and for directly and rapidly identifying Salm. typhimurium in food.
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