3‐amino‐1‐propanol as a complexing agent in the determination of total gossypol

Abstract

Summary A method is proposed for the determination of total gossypol in cottonseed meals, crude oils, and soapstocks based on a rapid extraction of gossypol by neutralized 3‐amino‐1‐propanol in dimethylformamide to form a stable complex, followed by colorimetric analysis of an aliquot of the extract by means of an aniline reaction. A determination can be completed in about 2 hrs. and with minor modification in 1 hr. compared to about 7 hrs. for current methods. Results obtained by the proposed procedure on meals, oils, and soapstocks are in essential agreement with those found by use of other accepted methods. Desirable features, such as stability of reagents and extracts and a high degree of reproducibility, suggest that the procedure will satisfy the requirements for a rapid and simplified method for the analysis of all cottonseed products for total gossypol.