Analytical Letters · 2007 · 27 citations · 11 references
MycotoxinsPreliminary StudyFood ToxicologyType‐a TrichothecenesHorseradish PeroxidaseBiosensing SystemsAflatoxin B1Financial SupportMycotoxin FormationBioanalysisAnalytical ChemistryToxicologyClinical ChemistryAnalytical BiotechnologyChromatographyHealth SciencesBiochemistryFood MycologyMicrobiologyMedicineDrug Analysis
Abstract In this work are presented methods for detection of aflatoxin B1 and type‐A trichothecenes, based on the use of indirect competitive ELISA format coupled with a 96‐well screen‐printed microplate. Electrochemical immunoassays for AFB1, T‐2, and HT‐2 were performed and the activity of the alkaline phosphatase or horseradish peroxidase labeled enzymes were measured using intermittent pulse amperometry (IPA) as electrochemical technique. Using standard solutions of the target analyte the LOD of the assays were 0.3 and 0.2 ng ml−1 for T‐2 and AFB1 respectively, while the sensitivity was 1.2 ng ml−1 for both. For Aflatoxin B1, a stability study of electrochemical plate was also performed. Moreover, the matrix effect was evaluated using two different extraction treatments from corn. Keywords: Aflatoxin B1 type‐A trichothecene96‐well screen‐printed microplatecompetitive ELISA The authors wish to thank the Italian projects MIPAF ‘Aflarid’, PRIN‐2005, the European project ‘Biocop’, and the EUROLAB S.r.l. for financial support.
11