A SIMPLE METHOD FOR THE DETERMINATION OF CHOLESTEROL AND SOME PLANT STEROLS IN FISHERY‐BASED FOOD PRODUCTS

Abstract

ABSTRACT An efficient method has been developed for the microdetermination of cholesterol and some plant sterols such as brassicasterol, campesterol, stigmasterol, and β‐sitosterol. The method is a greatly simplified analytical procedure in which samples are directly saponified, the unsaponifiable substances extracted, and the sterols estimated by gas liquid chromatography without further processing. The sterol contents from the new method are at least as high, alid generally higher, than those from the official method, indicating superior recovery. The analysis has been found to be simple, sensitive, economical of time and particularly of solvents. It is probably adaptable to a wide variety of food ingredients or products.