Concepedia

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Manfred T. Reetz, José Daniel Carballeira

Nature Protocols · 2007 · 866 citations · 44 references

DOI

Concepts

References

44

Protein production by auto-induction in high-density shaking cultures

F. William Studier · Protein Expression and Purification · 2005 · 6.1K citations

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Rapid evolution of a protein in vitro by DNA shuffling

Willem P.C. Stemmer · Nature · 1994 · 1.9K citations

, , +10

Methods in molecular biology

Philippe Métézeau · Research in Microbiology · 1998 · 1.3K citations

, , +8

Prediction of chain flexibility in proteins

P. Andrew Karplus, Georg E. Schulz · Die Naturwissenschaften · 1985 · 1.2K citations

, , +12

A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction

DW Leung, E Chen, Goeddel Dv · Medical Entomology and Zoology · 1989 · 612 citations

, , +13