Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) · 2010 · 22 citations · 14 references
AgricultureCrop SciencesGeneticsGermplasm EvaluationPlant GeneticsSeed BiotechnologyMolecular DiagnosisMolecular Markers In PlantsPolymerase Chain ReactionCommercial Seed DistributionEvent ScreeningGenomic DetectionMaize Variety AuthenticationMultiplex Detection AssaysMultiplex Nucleic Acid DetectionMultiplex Polymerase Chain Reaction (Pcr)Multiplex Polymerase Chain Reaction Assays
A novel multiplex PCR method was developed for simultaneous event-specific detection of four events of GM maize, i.e., DAS-59122-7, MIR604, MON88017, and MON863. The single laboratory examination of analytical performance using simulated DNA mixtures containing GM DNA at various concentrations in non-GM DNA suggested that the limits of detection (LOD) of the multiplex PCR method were 0.16% for MON863, MIR604, and MON88017, and 0.078% for DAS-59122-7. We previously developed a nonaplex (9plex) PCR method for eight events of GM maize, i.e., Bt11, Bt176, GA21, MON810, MON863, NK603, T25, and TC1507. Together with the nonaplex PCR method, the newly developed method enabled the detection and identification of eleven GM maize events that are frequently included in commercial GM seed used in Japan. In addition, this combinational analysis may be useful for the identification of combined event products of GM maize.
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