Science · 1993 · 63 citations · 32 references
Life SciencesImmunologyMolecular BiologyAdaptive ImmunityCellular ImmunologySignal TransductionCytotoxic T Lymphocyte ResponsesEffector T Cell FunctionT Cell BiologyT Cell DevelopmentT Cell DifferentiationThymic DevelopmentCd8 Positive T Cell DifferentiationCd8 Positive T CellsNegative SelectionNegative Selection Of T LymphocytesPositive SelectionPositive Selection Of T CellsThymic T Cell DevelopmentTransgenic Mice
The CD8 alpha cytoplasmic domain associates with p56lck, a nonreceptor protein-tyrosine kinase. The biological relevance of CD8 alpha-Lck association in T cell development was tested with transgenic mice generated to express a CD8 alpha molecule with two amino acid substitutions in its cytoplasmic domain, which abolishes the association of CD8 alpha with Lck. The CD8 alpha mutant was analyzed in a CD8-/- background and in the context of the transgenic 2C T cell receptor. The development and function of CD8+ T cells in these mice were apparently normal. Thus, CD8 alpha-Lck association is not necessary for positive selection, negative selection, or CD8-dependent cytotoxic function.
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Site-directed mutagenesis by overlap extension using the polymerase chain reaction
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