Identification of peptide-cross-linked trisdisaccharide peptide trimers in murein of Escherichia coli

Abstract

Purified murein from Escherichia coli K-12 was degraded into disaccharide peptide fragments by endo-N-acetylmuramidase from Chalaropsis. About 5% of the total murein fragments were recovered as peptide-cross-linked trisdisaccharide peptide trimers.