Leigh C. Bowering

A plasmid system for optimization of Fab′ production in Escherichia coli: importance of balance of heavy chain and light chain synthesis

David P. Humphreys, Bruce Carrington, Leigh C. Bowering et al. · Protein Expression and Purification · 2002 · 52 citations

Fab′ Production, Biosynthesis, Biomanufacturing +14

Alternative antibody Fab′ fragment PEGylation strategies: combination of strong reducing agents, disruption of the interchain disulphide bond and disulphide engineering

David P. Humphreys, Sam Heywood, Alistair J. Henry et al. · Protein Engineering Design and Selection · 2007 · 46 citations

Peg Size, Peptide Science, Chemical Biology +17

Fedbatch design for periplasmic product retention in Escherichia coli

Emma Bäcklund, Dominic G. Reeks, Katrin Markland et al. · Journal of Biotechnology · 2008 · 41 citations

Engineering, Fedbatch Design, Biochemical Engineering +4

Comparison of Techniques for Monitoring Antibody Fragment Production in <i>E. coli</i> Fermentation Cultures

Leigh C. Bowering, Daniel G. Bracewell, Eli Kesharvarz‐Moore et al. · Biotechnology Progress · 2002 · 21 citations

Engineering of Escherichia coli to improve the purification of periplasmic Fab′ fragments: changing the pI of the chromosomally encoded PhoS/PstS protein

David P. Humphreys, Sam Heywood, Lloyd King et al. · Protein Expression and Purification · 2004 · 13 citations

Engineering, Protein Expression, Protein Biosynthesis +10