Dominic G. Reeks

High-Level Periplasmic Expression in Escherichia coli Using a Eukaryotic Signal Peptide: Importance of Codon Usage at the 5′ End of the Coding Sequence

David P. Humphreys, Mukesh Sehdev, Andrew P. Chapman et al. · Protein Expression and Purification · 2000 · 67 citations

Codon Usage, Protein Expression, Natural Sciences +10

A plasmid system for optimization of Fab′ production in Escherichia coli: importance of balance of heavy chain and light chain synthesis

David P. Humphreys, Bruce Carrington, Leigh C. Bowering et al. · Protein Expression and Purification · 2002 · 52 citations

Fab′ Production, Biosynthesis, Biomanufacturing +14

Fedbatch design for periplasmic product retention in Escherichia coli

Emma Bäcklund, Dominic G. Reeks, Katrin Markland et al. · Journal of Biotechnology · 2008 · 41 citations

Engineering, Fedbatch Design, Biochemical Engineering +4

F(ab′)2 molecules made from Escherichia coli produced Fab′ with hinge sequences conferring increased serum survival in an animal model

David P. Humphreys, Olivia M. Vetterlein, Andrew P. Chapman et al. · Journal of Immunological Methods · 1998 · 34 citations

Protein Expression, Biochemistry, Natural Sciences +11

Efficient site specific removal of a C-terminal FLAG fusion from a Fab′ using copper(II) ion catalysed protein cleavage

David P. Humphreys, Bryan John Smith, Lloyd King et al. · Protein Engineering Design and Selection · 1999 · 22 citations

Protein Assembly, Cupric Ions, Molecular Biology +17