Continuous evolution of SpCas9 variants compatible with non-G PAMs

Shannon M. Miller, Tina Wang, Peyton B. Randolph, +7

Nature Biotechnology

Continuous EvolutionAllelic VariantG Protein-coupled ReceptorGeneticsComputational Biology +5

Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors

Tony P. Huang, Kevin T. Zhao, Shannon M. Miller, +5

Nature Biotechnology

EngineeringPam-modified Cas9 VariantsMolecular BiologySynthetic BiologyGene Editing +4

Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity

Monica E. Neugebauer, Alvin Hsu, Mandana Arbab, +11

Nature Biotechnology

CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA

Beverly Mok, Anna V. Kotrys, Aditya Raguram, +3

Nature Biotechnology

Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors

Jessie R. Davis, Xiao Wang, Isaac P. Witte, +7

Nature Biomedical Engineering

Precision genome editing using cytosine and adenine base editors in mammalian cells

Tony P. Huang, Gregory A. Newby, David R. Liu

Nature Protocols

ChromatinMammalian CellsMedicineNatural SciencesDna Replication +9

High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells

Hui Kwon Kim, Sungtae Lee, Younggwang Kim, +6

Nature Biomedical Engineering

BioinformaticsOff-target EffectHuman CellsNatural SciencesHigh-throughput Analysis +10

High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs

Tony P. Huang, Zachary Heins, Shannon M. Miller, +5

Nature Biotechnology

Continuous directed evolution of proteins with improved soluble expression

Tina Wang, Ahmed H. Badran, Tony P. Huang, +1

Nature Chemical Biology

Protein ExpressionProtein FoldingNatural SciencesDirected EvolutionProtein Evolution +3

Assigning functionality to cysteines by base editing of cancer dependency genes

Haoxin Li, Tiantai Ma, Jarrett R. Remsberg, +13

Nature Chemical Biology

Base EditingTumor BiologyOncogenic AgentMedicineNatural Sciences +11