Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

Genome engineering using the CRISPR-Cas9 system

F. Ann Ran, Patrick D. Hsu, Jason Wright et al. · Nature Protocols · 2013 · 11.4K citations · Full text

Engineering, Genome Surgery, Genetics +7

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali, Luhan Yang, Kevin M. Esvelt et al. · Science · 2013 · 9.2K citations · Full text

Engineering, Genetics, Molecular Biology +16

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench, Nicolò Fusi, Meagan E. Sullender et al. · Nature Biotechnology · 2016 · 4.7K citations · Full text

Systems Biology, Off-target Effect, Natural Sciences +10

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

Stan J. J. Brouns, Matthijs M. Jore, Magnus Lundgren et al. · Science · 2008 · 2.6K citations · Full text

Crispr Rnas, Viral Replication, Off-target Effect +13

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

Elitza Deltcheva, Krzysztof Chylinski, Cynthia M. Sharma et al. · Nature · 2011 · 2.5K citations · Full text

Natural Sciences, Genetics, Crispr Rna Maturation +9