Characteristics of fresh rice straw silage quality prepared with addition of lactic acid bacteria and crude cellulase

Abstract

<abstract> <p>The objective of this study was to determine the characteristics of fresh rice straw silage quality prepared with addition of <italic>Lactiplantibacillus plantarum</italic> 1A-2 and crude cellulase alone or in combination. Quality of the silage was observed through the chemical composition, chemical structure and <italic>in vitro</italic> digestibility. Six treatments were used in this study, i.e., 1) rice straw without any treatment as control, 2) rice straw with addition of 0.1% <italic>L. plantarum</italic> 1A-2 (LAB1), 3) rice straw with addition of 1% crude cellulase (E1), 4) rice straw with addition of 0.1% <italic>L. plantarum</italic> 1A-2 and 1% cellulase enzyme. (LAB1 E1), 5) rice straw with addition of 2% crude cellulase (E2), 6) rice straw with addition of 0.2% <italic>L. plantarum</italic> 1A-2 and 2% crude cellulase (LAB2E2). Each treatment was replicated by four times (n = 24). Ensilage was carried out for 60 days. Data obtained were analyzed by using one-way analysis of variance (ANOVA) according to complete randomized design. The result indicated that the treatments increased dry matter (DM) (p = 0.001), crude protein (p < 0.001) and lactic acid (p < 0.001). Meanwhile, reduced pH (p < 0.001) and organic acids (acetic, propionic and butyric (p < 0.001)). Total crystallinity index (TCI) of rice straw silage varied among treatments and decreased in crystallinity (%) except for LAB2E2, which showed the lowest crystalline size. The treatment increased DM digestibility (p = 0.397) with the highest in LAB2E2. There is significant effect (p < 0.001) on increasing the main SCFA products from <italic>in vitro</italic> rumen fermentation. This study suggests that addition of <italic>L. plantarum</italic> 1A-2 inoculant alone or with crude cellulase improved fresh rice straw silage quality.</p> </abstract>