Potential of Volatile Organic Compounds Emitted by <i>Pseudomonas fluorescens</i> ZX as Biological Fumigants to Control Citrus Green Mold Decay at Postharvest

Abstract

In this study, volatile organic compounds (VOCs) were generated by <i>Pseudomonas fluorescens</i> ZX with incubation in nutrient broth (NB), on NA (NB with agar), and on healthy orange fruits, and pure individual components of VOCs were used to manage citrus green mold infected by <i>Penicillium digitatum</i>. At a concentration of 1 × 10¹⁰ cfu/mL, the VOCs from antagonist-containing NA plates inhibited <i>P. digitatum</i> conidial germination and mycelial growth by about 60%, while the VOCs from bacterial fluid exhibited approximately 75% inhibitory effect. Biofumigation by VOCs significantly reduced the disease index, with a higher biocontrol efficacy by VOCs from bacterial fluid (about 51%) than from antagonist-containing NA plates (around 40%) or from antagonist-infested fruit (approximately 24%). Exposure to VOCs led to morphological abnormalities of <i>P. digitatum</i> conidia and hyphae. However, VOCs exhibited poor preventative and curative action against <i>P. digitatum</i>. The storage test showed that biofumigation had no negative effects on fruit quality. Antifungal assays suggested that dimethyl disulfide and dimethyl trisulfide exhibited the highest inhibitory effects, which afforded complete inhibition at the lowest concentrations. In addition, organic acids were also promising in controlling green mold, but only at suitable low concentrations to avoid eliciting fruit's physiological diseases.