Rational engineering of transcriptional riboswitches leads to enhanced metabolite levels in Bacillus subtilis

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench, Nicolò Fusi, Meagan E. Sullender et al. · Nature Biotechnology · 2016 · 5K citations

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A One Pot, One Step, Precision Cloning Method with High Throughput Capability

Carola Engler, Romy Kandzia, Sylvestre Marillonnet · PLoS ONE · 2008 · 2.6K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

Sangsu Bae, Jeongbin Park, Jin‐Soo Kim · Bioinformatics · 2014 · 2.4K citations · Full text

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NUPACK: Analysis and design of nucleic acid systems

Joseph N. Zadeh, Conrad Steenberg, Justin S. Bois et al. · Journal of Computational Chemistry · 2010 · 1.8K citations · Full text

Life Sciences, Bioinformatics, Biophysics +17

Lambda replacement vectors carrying polylinker sequences

Anna‐Maria Frischauf, Hans Lehrach, Annemarie Poustka et al. · Journal of Molecular Biology · 1983 · 1.8K citations

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