Evolution of plant mutagenesis tools: a shifting paradigm from random to targeted genome editing

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jínek, Krzysztof Chylinski, Ines Fonfara et al. · Science · 2012 · 16.7K citations · Full text

Crispr Rnas, Adaptive Bacterial Immunity, Crispr-associated Protein Cas9 +15

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

Andrew Fire, Siqun Xu, Mary K. Montgomery et al. · Nature · 1998 · 15.1K citations

Specific Genetic Interference, Natural Sciences, Genetics +9

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

Rodolphe Barrangou, Christophe Fremaux, Hélène Deveau et al. · Science · 2007 · 6.2K citations

Phage-resistance Phenotype, Genetics, Bacteriophage +12

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Alexis C. Komor, Y. Bill Kim, Michael S. Packer et al. · Nature · 2016 · 5.2K citations · Full text

Engineering, Genetics, Synthetic Biology +11

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench, Nicolò Fusi, Meagan E. Sullender et al. · Nature Biotechnology · 2016 · 4.7K citations · Full text

Systems Biology, Off-target Effect, Natural Sciences +10