Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

Ophir Shalem, Neville E. Sanjana, Ella Hartenian, +8

Science

EngineeringGeneticsGecko LibraryTumor BiologyHuman Cells +14

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Silvana Konermann, Mark D. Brigham, Alexandro E. Trevino, +9

Nature

Engineered Crispr-cas9 ComplexTranscriptional RegulationNatural SciencesGenetic EngineeringMolecular Biology +6

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

Luke A. Gilbert, Max A. Horlbeck, Britt Adamson, +10

Cell

Transcriptional RegulationGeneticsGenetic EngineeringGene RepressionGene Editing +5

MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

Wei Li, Han Xu, Tengfei Xiao, +7

Genome biology

EngineeringGeneticsMolecular BiologyGenomicsEssential Genes +17

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

Baohui Chen, Luke A. Gilbert, Beth A. Cimini, +8

Cell

Optimized Crispr/cas SystemDynamic ImagingGenomic LociMolecular BiologyGenetic Engineering +6

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

Atray Dixit, Oren Parnas, Biyu Li, +13

Cell

Functional GenomicsPooled Genetic ScreensMedicineGeneticsSingle Cell Sequencing +12

Identification and characterization of essential genes in the human genome

Kıvanç Birsoy, Nicholas W. Hughes, Kevin M. Krupczak, +4

Science

Robust rank aggregation for gene list integration and meta-analysis

Raivo Kolde, Sven Laur, Priit Adler, +1

Bioinformatics

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

Britt Adamson, Thomas M. Norman, Marco Jost, +14

Cell

Genome SurgerySingle Cell SequencingMedicineUnfolded Protein ResponseMolecular Biology +6

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

Hiroko Koike-Yusa, Yilong Li, E‐Pien Tan, +2

Nature Biotechnology

MedicineGeneticsGenetic EngineeringGene EditingGenome Editing +4