Journal of Biological Chemistry · 2019 · 25 citations · 33 references
Life SciencesBiotechnologyMolecular BiologyDna RepairGenome EditingProteomicsCrispr-based EngineeringCrispr-cas SystemsAntibodiesCrispr-cas Genome EditingCrispr-cas9 Genome EditingCrispr-cas9-mediated RepairDna End JoiningMicrohomology-mediated End JoiningMicrohomology-mediated Repair
, mCherry, GFP, and the auxin-inducible degron tag for compound-induced protein depletion. This approach and the developed tools should greatly facilitate functional analysis of proteins in their native environment.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
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Genome engineering using the CRISPR-Cas9 system
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Enzymatic assembly of DNA molecules up to several hundred kilobases
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An auxin-based degron system for the rapid depletion of proteins in nonplant cells
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