Concepedia

Significantly increased catalytic activity of<i>Candida antarctica</i>lipase B for the resolution of<i>cis</i>-(±)-dimethyl 1-acetylpiperidine-2,3-dicarboxylate

Jiang-Wei Shen, Jia-Mei Qi, Xiaojian Zhang, Zhi‐Qiang Liu, Yu‐Guo Zheng

Catalysis Science & Technology · 2018 · 25 citations · 33 references

DOI

Concepts

Abstract

Structure-based semi-rational engineering approach was applied to alter the binding pocket and substrate channel for enhancing the activity of CALB towards moxifloxacin chiral intermediate.

References

33

AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading

Oleg Trott, Arthur J. Olson · Journal of Computational Chemistry · 2009 · 37.1K citations · Full text

, , +17

PROCHECK: a program to check the stereochemical quality of protein structures

Roman A. Laskowski, Malcolm W. MacArthur, D. S. Moss et al. · Journal of Applied Crystallography · 1993 · 24.6K citations

, , +9

An efficient one-step site-directed and site-saturation mutagenesis protocol

Lei Zheng · Nucleic Acids Research · 2004 · 1.2K citations · Full text

, , +15

The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica

J. Uppenberg, Mogens Trier Hansen, Shamkant Patkar et al. · Structure · 1994 · 903 citations

, , +12

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Manfred T. Reetz, José Daniel Carballeira · Nature Protocols · 2007 · 866 citations

, , +15