Determination of Trans-10-Hydroxy-2-Decenoic Acid in Royal Jelly by High Performance Liquid Chromatography after Different Bee Feeding

Abstract

Royal jelly (RJ) is a bee product with great potential as a supplementary food and in the future it will be very important for the pharmaceutical industry. The aim of this study is first to determine the content of 10-HDA in Bulgarian RJ and second to identify if the content of 10-HDA in RJ depends on supplementary feeding of the bee families. A total of 50 RJ samples were analyzed. The data about Bulgarian royal jelly is not available, therefore the results presented in this study could be considered as a pioneer study for 10-HDA content in Bulgarian royal jelly. Range (1.64 – 2.32%) for 10-HDA in RJ samples received from the control group bee families could be used as a first proposal for Bulgarian standard for 10-HDA. Furthermore, this is the first data for significantly higher content of 10-HDA in RJ samples after feeding of the bee families with baker’s yeast (p<0.01) compare to the control group bee families.