Gibson Assembly facilitates bacterial allelic exchange mutagenesis

Enzymatic assembly of DNA molecules up to several hundred kilobases

Daniel G. Gibson, Lei Young, Ray-Yuan Chuang et al. · Nature Methods · 2009 · 10.9K citations

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Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

M J Casadaban, Stanley N. Cohen · Journal of Molecular Biology · 1980 · 2.5K citations

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RNA-guided editing of bacterial genomes using CRISPR-Cas systems

Wenyan Jiang, David Bikard, David Cox et al. · Nature Biotechnology · 2013 · 2.5K citations

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Gene splicing and mutagenesis by PCR-driven overlap extension

Karin L. Heckman, Larry R. Pease · Nature Protocols · 2007 · 1.2K citations

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Recombineering: a homologous recombination-based method of genetic engineering

Shyam K. Sharan, Lynn C. Thomason, Sergey G. Kuznetsov et al. · Nature Protocols · 2009 · 823 citations · Full text

Life Sciences, Biotechnology, Genetics +14