Concepedia

Gene splicing and mutagenesis by PCR-driven overlap extension

Karin L. Heckman, Larry R. Pease

Nature Protocols · 2007 · 1.2K citations · 21 references

DOI

Concepts

References

21

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

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Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

Robert M. Horton, Henry D. Hunt, Steffan N. Ho et al. · Gene · 1989 · 3.1K citations

, , +10

The "megaprimer" method of site-directed mutagenesis.

Gobinda Sarkar, S S Sommer · PubMed · 1990 · 1.2K citations

, , +12

Current Protocols in Molecular Biology

Tim Hunt · Journal of Cell Science · 1989 · 969 citations

, , +10

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

Mark J. Zoller, Michael J. Smith · Nucleic Acids Research · 1982 · 762 citations · Full text

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