Concepedia

Pcr-based Mutagenesis

PCR-Based Mutagenesis is a molecular biology technique that belongs to the class of site-directed mutagenesis methods, distinguished by its use of polymerase chain reaction amplification to introduce specific nucleotide changes at targeted genomic loci through the incorporation of mutagenic primers.

424

Publications

40.9K

Citations

1.5K

Authors

473

Institutions

Publications per year

2017–2026

16

Variants

Pcr-mediated Mutagenesis

Parents

Publications

424

A general method of<i>in vitro</i>preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

Russell Higuchi, Barbara Krummel, Randall K. Saiki · Nucleic Acids Research · 1988 · 2.4K citations · Full text

, , +12

Heterologous modules for efficient and versatile PCR-based gene targeting inSchizosaccharomyces pombe

Jürg Bähler, Jian‐Qiu Wu, Mark S. Longtine et al. · Yeast · 1998 · 2.3K citations

, , +17

Bias in Template-to-Product Ratios in Multitemplate PCR

Martin F. Polz, Colleen M. Cavanaugh · Applied and Environmental Microbiology · 1998 · 1.5K citations · Full text

, , +14

An efficient one-step site-directed and site-saturation mutagenesis protocol

Lei Zheng · Nucleic Acids Research · 2004 · 1.2K citations · Full text

, , +15

The "megaprimer" method of site-directed mutagenesis.

Gobinda Sarkar, S S Sommer · PubMed · 1990 · 1.2K citations

, , +12

1–5 of 424

Authors

1.5K

  1. RC
    Roger C. Lévesque

    Université Laval, Canada

    5 publications · 140 citations

    5

    h-index

  2. SB
    Sailen Barik

    University of South Alabama, United States

    4 publications · 239 citations

    4

    h-index

  3. LN
    L. Nicholas Ornston

    Yale University, United States

    3 publications · 106 citations

    3

    h-index

  4. MW
    Miles Wilkinson

    The University of Texas MD Anderson Cancer Center, United States

    3 publications · 184 citations

    3

    h-index

  5. SB
    Sergei B. Vakulenko

    University of Notre Dame, United States

    3 publications · 118 citations

    3

    h-index

1–5 of 1.5K

Institutions

473

  1. Harvard University

    Cambridge, United States

    10 h-index

    19 publications

    5.1K citations

  2. University of Iowa

    Iowa City, United States

    7 h-index

    14 publications

    531 citations

  3. Howard Hughes Medical Institute

    Chevy Chase, United States

    6 h-index

    15 publications

    1.5K citations

  4. University of Wisconsin–Madison

    Madison, United States

    5 h-index

    18 publications

    782 citations

  5. Université Laval

    Québec, Canada

    5 h-index

    16 publications

    362 citations

  6. RIKEN Advanced Science Institute

    Kobe, Japan

    5 h-index

    15 publications

    1.1K citations

  7. The University of Texas MD Anderson Cancer Center

    Houston, United States

    5 h-index

    10 publications

    511 citations

  8. Yale University

    New Haven, United States

    4 h-index

    15 publications

    522 citations

1–8 of 473

Venues

  1. Nucleic Acids Research

    41 publications · 8.9K citations

    32

    h-index

  2. PubMed

    Repository · 32 publications · 2.5K citations

    21

    h-index

  3. BioTechniques

    28 publications · 2.8K citations

    20

    h-index

  4. Journal of Bacteriology

    JB · 16 publications · 721 citations

    14

    h-index

  5. Humana Press eBooks

    Ebook platform · 13 publications · 763 citations

    13

    h-index

1–5