Concepedia

Gibson Assembly

Gibson Assembly is a molecular cloning technique that enables seamless DNA assembly by combining exonuclease, DNA polymerase, and DNA ligase activities in a single isothermal reaction to join multiple overlapping DNA fragments with high efficiency and precision.

41

Publications

1.7K

Citations

221

Authors

97

Institutions

Publications per year

2017–2026

21

Parents

Publications

41

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

Florian Weissmann, Georg Petzold, Ryan T. VanderLinden et al. · Proceedings of the National Academy of Sciences · 2016 · 414 citations · Full text

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A rapid seamless method for gene knockout in Pseudomonas aeruginosa

Weiliang Huang, Angela Wilks · BMC Microbiology · 2017 · 91 citations · Full text

, , +16

CRISPR/Cas9 Nuclease Cleavage Combined with Gibson Assembly for Seamless Cloning

Jiawang Wang, Amy Wang, Kunyu Li et al. · BioTechniques · 2015 · 82 citations · Full text

, , +15

CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA

Jianting Zhou, Ronghai Wu, Xiaoli Xue et al. · Nucleic Acids Research · 2016 · 78 citations · Full text

, , +17

Rapid cloning, expression, and functional characterization of paired αβ and γδ T-cell receptor chains from single-cell analysis

Xi-zhi J. Guo, Pradyot Dash, Matthew Calverley et al. · Molecular Therapy — Methods & Clinical Development · 2016 · 64 citations · Full text

, , +14

1–5 of 41

Authors

221

  1. RM
    Ruth M. Hall

    The University of Sydney, Australia

    2 publications · 49 citations

    2

    h-index

  2. RC
    Rex C. Haydon

    University of Chicago Medical Center, United States

    2 publications · 79 citations

    2

    h-index

  3. TH
    Tong‐Chuan He

    University of Chicago Medical Center, United States

    2 publications · 79 citations

    2

    h-index

  4. RR
    Russell R. Reid

    University of Chicago Medical Center, United States

    2 publications · 79 citations

    2

    h-index

  5. JW
    James W. Ajioka

    University of Cambridge, United Kingdom

    2 publications · 41 citations

    2

    h-index

1–5 of 221

Institutions

97

  1. Chinese Academy of Sciences

    Beijing, China

    4 h-index

    9 publications

    478 citations

  2. University of Edinburgh

    Edinburgh, United Kingdom

    3 h-index

    9 publications

    364 citations

  3. University of Tennessee Health Science Center

    Memphis, United States

    2 h-index

    3 publications

    542 citations

  4. Army Medical University

    Chongqing, China

    2 h-index

    3 publications

    135 citations

  5. University of Chicago Medical Center

    Chicago, United States

    2 h-index

    45 publications

    1.8K citations

  6. Shanghai Institutes for Biological Sciences

    Shanghai, China

    2 h-index

    7 publications

    411 citations

  7. University of Cambridge

    Cambridge, United Kingdom

    2 h-index

    9 publications

    222 citations

  8. St. Jude Children's Research Hospital

    Memphis, United States

    2 h-index

    9 publications

    1.6K citations

1–8 of 97

Venues

  1. PLoS ONE

    6 publications · 189 citations

    6

    h-index

  2. ACS Synthetic Biology

    4 publications · 113 citations

    4

    h-index

  3. Virology Journal

    2 publications · 63 citations

    2

    h-index

  4. Journal of Microbiological Methods

    2 publications · 32 citations

    2

    h-index

  5. Methods in molecular biology

    Book series · 2 publications · 62 citations

    2

    h-index

1–5