Site-directed mutagenesis of GH10 xylanase A from Penicillium canescens for determining factors affecting the enzyme thermostability

Ligation-independent cloning of PCR products (LIC-PCR)

Charalampos Aslanidis, Pieter J. de Jong · Nucleic Acids Research · 1990 · 1.2K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +14

An additional aromatic interaction improves the thermostability and thermophilicity of a mesophilic family 11 xylanase: Structural basis and molecular study

Jacques Georis, Frédéric De Lemos Esteves, Josette Lamotte‐Brasseur et al. · Protein Science · 2000 · 173 citations · Full text

Life Sciences, Microbiology, Molecular Biology +17

Engineering Hyperthermostability into a GH11 Xylanase Is Mediated by Subtle Changes to Protein Structure

Claire Dumon, Alexander Varvak, M. A. Wall et al. · Journal of Biological Chemistry · 2008 · 125 citations · Full text

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Thermostability Improvement of a Streptomyces Xylanase by Introducing Proline and Glutamic Acid Residues

Kun Wang, Huiying Luo, Jian Tian et al. · Applied and Environmental Microbiology · 2014 · 121 citations · Full text

Life Sciences, Biotechnology, Microbiology +17

A combination of weakly stabilizing mutations with a disulfide bridge in the α-helix region of Trichoderma reesei endo-1,4-β-xylanase II increases the thermal stability through synergism

Ossi Turunen, K. Etuaho, Fred Fenel et al. · Journal of Biotechnology · 2001 · 116 citations

Life Sciences, Biotechnology, Molecular Biology +17