The use of pancreatic lipase for determining the distribution of fatty acids in partial and complete glycerides

Abstract

The method of digestion with pancreatic lipase for determining the position of fatty acids in triglycerides has been modified to increase its accurary and ease of applicability. Moreover, by acylation of partial glycerides with a “marker” fatty acid, the method can be used for determining the structure of mono- and diglycerides. Evidence is presented which demonstrates that acylation of partial glycerides with fatty acid chlorides can be carried out without causing rearrangement of the partial glycerides.