A Method for Extracting Intact RNA from Fruits Rich in Polysaccharides using Ripe Mango Mesocarp

Abstract

A rapid and simple procedure is described for efficiently extracting intact RNA from mango (Mangifera indica L. cv. Manila) mesocarp, a tissue rich in polysaccharides. The RNA can be used for in vitro translation, northern blots, and cDNA synthesis. This method is applicable to other fleshy fruits rich in polysaccharides.