FREE RADICAL SCAVENGING ACTIVITY AND ANTIOXIDANT CAPACITY OF ERYNGIUM CAUCASICUM TRAUTV AND FRORIPIA SUBPINNATA

Abstract

Eryngium caucasicum Trautv and Froriepia subpinnata were found in natively in the eastern parts of the south Caspian Sea coasts. Antioxidant activity of E. caucasicum Trautv and F. subpinnata were investigated employing six in vitro assay systems. IC50 for DPPH radical-scavenging activity was 0.27 ± 0.02 for E. caucasicum leaves and 0.42 ± 0.03 mg ml-1 for F. subpinnata, respectively. The extracts showed weak nitric oxide-scavenging and Fe2+ chelating ability activity. The IC50 of E. caucasicum was better than other one with IC50= 0.21 mg ml-1 in NO scaveging test. The peroxidation inhibition of E. caucasicum extract exhibited values from 93 (at 24th) to 97% (at 72nd hrs). F. subpinnata exhibited very low antioxidant activity. The activity of E. caucasicum was comparable with Vit C (p> 0.05). No extract showed good scavenging activity of H2O2. The total amount of phenolic compounds in each extracts was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Both of them had high total phenolic and flavonoid contents.