Estimation of Flavonoid, Polyphenolic Content and In-vitro Antioxidant Capacity of leaves of Tephrosia purpurea Linn. (Leguminosae)

Abstract

Introduction Leaves of Tephrosia purpurea Linn (sarpankh), belonging to the family Leguminosae being used for the treatment of jaundice and claimed to be effective in many other diseases. The present research work was under taken to investigate the in-vitro antioxidant activity of aqueous and ethanolic extracts. Method The therapeutic effects of tannins and flavonoids can be largely attributed to their antioxidant properties. So that the quantitative determinations were undertaken. All the methods are based on UV-Spectrophotometric determination. Result The total Phenolic content of aqueous and ethanolic extracts showed the content values of 9.44 ± 0.22% w/w and 18.44 ± 0.13% w/w and total flavonoids estimation of aqueous and ethanolic extract showed the content values of 0.91 ± 0.08 % w/w and 1.56 ± 0.12%w/w for Quercetin and for 1.85 ± 0.08 % w/w and 2.54 ± 0.12 % w/w Rutin respectively. Further investigation were carried out for In-vitro antioxidant activity and Radical scavenging assay by calculating its % inhibition by means of IC50 values, all the extracts concentration has been adjusted to come under the linearity range and here many reference standards like Tannic acid, Gallic acid, Quercetin, Ascorbic acid have been taken for the method suitability. Conclusion The results revealed that leaves of this plant have antioxidant potential .Among these results ethanolic extract has more potent than traditionally claiming aqueous decoction. In conclusion that Tephrosia purpurea Linn. (Leguminosae) leaves possesses the antioxidant substance which may be potential responsible for the