Incidence of the Most Common Toxigenic Aspergillus Species in Broiler Feeds in Kermanshah Province, West of Iran

Abstract

Abstract: The objectives of this study were to investigate the occurrence and identification of Aspergillus species in broiler feeds in Kermanshah province, west of Iran. From April 2008 to March 2009, a total of 50 samples of broiler feeds were collected from different broiler farms located in Kermanshah province. All samples were aseptically transported to the laboratory, homogenized, quartered to obtain a 1 kg laboratory sample and were stored at 4°C for fungal analyses. Ten grams of each feed sample were homogenized in 90 ml sterile physiological saline for 30 minutes to obtain a concentration of 10 (dilution 1). This mixture1 was then serially diluted to 10 (dilution 2), 10 (dilution 3) and 10 (dilution 4). From each dilution, 25 µL of2 3 4 mixture was deeply inoculated on dichloran rose-bengal-chloranphenicol agar (DRBC) and incubated at 30°C for 15 days. During incubation period, gross and microscopic features of fungal colonies were studied. Out of